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KMID : 1059519980420020209
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Journal of the Korean Chemical Society
1998 Volume.42 No. 2 p.209 ~ p.213
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Analysis of Higher Order Structure of 5S rRNA from Pseudomonas Alcaligens Byusing (η6-mesitylene) manganese-(I) Tricabonyl hexafluorophosphate
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Kim Sang-Bumn
Park In-Won
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Abstract
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The higher order structure of Pseudomonas alcaligenes 5S rRNA has been investigated by using (η6-mesitylene) manganese (Ⅰ) tricarbonyl hexafluorophosphate[MTH-Mn (Ⅰ)], dimethylpyrocarbonate, potassium permanganate as chemical probes. The sequences cleaved strongly by MTH-Mn (Ⅰ) on the tertiary structure of the 5S rRNA are G12AUGG16 of loop a, G51AAGUGAAGC60 of the region b-C, U65-AGCG69 of the region B-a, and G72AUGG76 of loop d. Based on such cleavage patterns of 5S rRNA by MTH-Mn(Ⅰ) and other chemical probes, we presume that the sequences strongly cleaved form pocket-like structure as in the corner of L structure of tRNAPhe. We also presume that the region b-C and loop d together play a role of hinge in forming the pocket-like structure in the 5S rRNA.
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KEYWORD
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